The lack of extracellular Na+ exacerbates Ca2+-dependent damage of cultured cerebellar granule cells
Identifieur interne : 000E30 ( Main/Exploration ); précédent : 000E29; suivant : 000E31The lack of extracellular Na+ exacerbates Ca2+-dependent damage of cultured cerebellar granule cells
Auteurs : Nikolaj K. Isaev [Russie] ; Elena V. Stelmashook [Russie] ; Olga P. Alexandrova [Russie] ; Nadezhda A. Andreeva [Russie] ; Irina A. Polyakova [Russie] ; Ilya V. Victorov [Russie] ; Dmitry B. Zorov [Russie]Source :
- FEBS Letters [ 0014-5793 ] ; 1998.
Abstract
Rhodamine 123 staining, light and electron microscopy were used to evaluate the ultrastructural and functional state of cultured cerebellar granule cells after short treatment with the solution where NaCl was substituted by sucrose (sucrose balance salt medium, SBSM). Cell exposure to SBSM for 20 min resulted in the fact that mitochondria in the neurons lost their ability to sequester rhodamine 123. This effect could be prevented by: (i) non-competitive N-methyl-d-aspartate (NMDA) receptor channel blocker, 10−5 M MK-801; (ii) a competitive specific antagonist of NMDA glutamate receptors, 0.25×10−3 M d,l-2-amino-7-phosphonoheptanoate (APH); (iii) 10−3 M cobalt chloride; (iv) removal of Ca2+ from the medium. Low Na+ in the Ca2+-containing medium caused considerable mitochondrial swelling in granule cells. However, the same treatment in the absence of calcium ions in the medium abolished the deleterious effect of SBSM on the neuronal mitochondrial structure and functions. It is suggected that (i) the exposure of cultured cerebellar granule cells to SBSM leads to a release of endogenous glutamate from cells; (ii) Ca2+ ions potentially de-energizing neuronal mitochondria enter the neuron preferentially through the NMDA channels rather than through the Na+/Ca2+ exchanger; (iii) mitochondrial swelling in granule cells is highly Ca2+-dependent; (iv) cellular overload with sodium ions can activate mitochondrial Na+/Ca2+ exchanger and thus prevent permeability transition pore opening in mitochondria.
Url:
DOI: 10.1016/S0014-5793(98)00979-X
Affiliations:
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Cell exposure to SBSM for 20 min resulted in the fact that mitochondria in the neurons lost their ability to sequester rhodamine 123. This effect could be prevented by: (i) non-competitive N-methyl-d-aspartate (NMDA) receptor channel blocker, 10−5 M MK-801; (ii) a competitive specific antagonist of NMDA glutamate receptors, 0.25×10−3 M d,l-2-amino-7-phosphonoheptanoate (APH); (iii) 10−3 M cobalt chloride; (iv) removal of Ca2+ from the medium. Low Na+ in the Ca2+-containing medium caused considerable mitochondrial swelling in granule cells. However, the same treatment in the absence of calcium ions in the medium abolished the deleterious effect of SBSM on the neuronal mitochondrial structure and functions. It is suggected that (i) the exposure of cultured cerebellar granule cells to SBSM leads to a release of endogenous glutamate from cells; (ii) Ca2+ ions potentially de-energizing neuronal mitochondria enter the neuron preferentially through the NMDA channels rather than through the Na+/Ca2+ exchanger; (iii) mitochondrial swelling in granule cells is highly Ca2+-dependent; (iv) cellular overload with sodium ions can activate mitochondrial Na+/Ca2+ exchanger and thus prevent permeability transition pore opening in mitochondria.</div></front></TEI><affiliations><list><country><li>Russie</li></country><region><li>District fédéral central</li></region><settlement><li>Moscou</li></settlement><orgName><li>Université d'État de Moscou</li></orgName></list><tree><country name="Russie"><region name="District fédéral central"><name sortKey="Isaev, Nikolaj K" sort="Isaev, Nikolaj K" uniqKey="Isaev N" first="Nikolaj K" last="Isaev">Nikolaj K. Isaev</name></region><name sortKey="Alexandrova, Olga P" sort="Alexandrova, Olga P" uniqKey="Alexandrova O" first="Olga P" last="Alexandrova">Olga P. Alexandrova</name><name sortKey="Andreeva, Nadezhda A" sort="Andreeva, Nadezhda A" uniqKey="Andreeva N" first="Nadezhda A" last="Andreeva">Nadezhda A. Andreeva</name><name sortKey="Polyakova, Irina A" sort="Polyakova, Irina A" uniqKey="Polyakova I" first="Irina A" last="Polyakova">Irina A. Polyakova</name><name sortKey="Stelmashook, Elena V" sort="Stelmashook, Elena V" uniqKey="Stelmashook E" first="Elena V" last="Stelmashook">Elena V. Stelmashook</name><name sortKey="Victorov, Ilya V" sort="Victorov, Ilya V" uniqKey="Victorov I" first="Ilya V" last="Victorov">Ilya V. Victorov</name><name sortKey="Zorov, Dmitry B" sort="Zorov, Dmitry B" uniqKey="Zorov D" first="Dmitry B" last="Zorov">Dmitry B. Zorov</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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